Allison Judge

Biochemistry PhD with 9 years of experience in the lab. As a postdoctoral fellow in Dr. Lauren Colbert's lab, I work as part of a team developing microbiome-based therapeutics for cervical cancer treatment. I have drug discovery experience in the structure-based design of small molecules and drug development experience in peptide-based therapeutics. Other technical skills include NGS library prep and data analysis, Python, R, enzymology, X-ray crystallography, mammalian cell culture, qPCR, and protein expression/purification.

• NIH T32 Fellow, Training Interdisciplinary Pharmacology Scientists (TIPS)
• Lehmann Outstanding Graduate Student Award

• A drug-resistant β-lactamase variant changes the conformation of its active-site proton shuttle to alter substrate specificity and inhibitor potency
• Supercoiling and looping promote DNA base accessibility and coordination among distant sites
• An active site loop toggles between conformations to control antibiotic hydrolysis and inhibition potency for CTX-M β-lactamase drug-resistance enzymes
• Network of epistatic interactions in an enzyme active site revealed by large-scale deep mutational scanning
• Mapping the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M β-lactamase