Summary
Overview
Work History
Education
Skills
Websites
References
Publications
Timeline
Generic

Manoj Gaddam

Burnsville

Summary

Skilled clinical laboratory technician with hands-on experience in DNA extraction, PCR analysis, and reagent preparation. Enhancing laboratory operations and maintaining compliance through precise analytical techniques, and clear, effective scientific documentation.

Overview

3
3
years of professional experience

Work History

Clinical Laboratory Technician II (CLIA-certified)

Valley Medical and Wellness
Burnsville
10.2024 - Current
  • Collected and processed urine specimens for toxicology analysis, including targeted testing for pain management, and EtG (alcohol biomarker) detection.
  • Reagent preparation for toxicology: proficient in formulating reagents required for toxicology testing, ensuring accuracy, and compliance with safety standards.
  • Operated LCMS systems: Loaded and managed plates on LCMS (Liquid Chromatography-Mass Spectrometry) for advanced substance analysis.
  • Performed creatinine testing: Conducted creatinine tests on the Architect analyzer to assess kidney function and sample validity.
  • DNA Extraction for Human Identification: Experienced in DNA extraction protocols for human identification processes.
  • Preparation of Molecular Reagents: Adept at preparing essential molecular biology reagents, including primers and probes for various applications.
  • PCR and GEXP Techniques: Demonstrated capability in utilizing gene expression profiling (GEXP) and performing PCR setup and loading, enhancing laboratory efficiency and data accuracy.

Microbiologist

Transparent Testing, LLC.
Tulsa
04.2024 - 09.2024
  • Conducted microbiological analysis of Cannabis spp. Flowers to identify and quantify microbial contaminants.
  • Performed DNA extractions from cannabis flowers and marijuana-derived products for downstream molecular applications.
  • Tested cannabis flower samples for residual pesticide contamination in compliance with regulatory standards.
  • Cultured and plated flower extracts to isolate and identify microbial species, including mold, and bacterial pathogens.
  • Experienced in handling and analyzing microbial contaminants, such as E. Coli, Aspergillus spp., and various molds.
  • Utilized molecular biology techniques, including qPCR (AriaMx Real-Time PCR) and RT-PCR, for pathogen detection and quantification.
  • Maintained and reported analytical results in compliance with the Oklahoma Medical Marijuana Authority (OMMA) regulatory requirements.

Quality Control Laboratory Technician

Laird Family Estate
Napa
09.2023 - 03.2024
  • Performed comprehensive chemical and microbiological analyses on multiple wine samples, including Brix, pH, titratable acidity (TA), volatile acidity (VA), malic acid, residual sugar (enzymatic), NOPA, ammonia, free and total SO₂, specific gravity (SG), dissolved oxygen (DO), cold and heat stability, turbidity, ethanol concentration, Brettanomyces detection, and bottling sterility.
  • Conducted yeast inoculations, nutrient additions, and routinely monitored and analyzed fermentation progress to ensure optimal wine quality.
  • Supported administrative tasks, including the preparation of reports, invoices, and accurate cataloging of production and laboratory records.
  • Applied standard research methodologies to routine laboratory tasks, while maintaining meticulous records, and ensuring data integrity.

Graduate Research Assistant

Texas A&M University-Kingsville Citrus Center
Weslaco
01.2022 - 08.2023
  • Field scouting for citrus pests and diseases, especially HLB affected trees.
  • Foliar Antimicrobial Peptides (AMPs) application to citrus trees.
  • Processing leaf samples and phloem sap collection for downstream testing of mineral nutrient and amino acid content
  • Experienced working in a Biosafety level (BSL)-1 laboratory
  • Designed and conducted experiments, collected data, and maintained results databases.
  • DNA & RNA extractions from leaf and root tissues
  • Performed various molecular techniques such as quantitative polymerase chain reaction(qPCR), conventional PCR, RT-PCR, Gel-electrophoresis, ELISA, DNA cloning, and cDNA to amplify the target DNA.
  • Worked on E. coli transformations, culturing bacteria, and extracting plasmid DNA.
  • Assessed the CLas titer using qPCR data from DNA samples taken from root and leaf tissue as well as from RNA extractions following the application of peptides.

Education

MS - Plant and Soil Sciences

Texas A&M University-Kingsville
Kingsville, TX
08.2023

Bachelor of Science - Agriculture (Honours)

Lovely Professional University
Phagwara, Punjab, India
08.2020

Skills

  • DNA and RNA extractions from plant cells
  • DNA Extraction for Human Identification
  • Urine extraction for pain management and EtG (alcohol biomarker) detection
  • Quantitative polymerase chain reaction (qPCR)
  • Conventional PCR, RT-PCR
  • Gel electrophoresis, Gene expression profiling (GEXP)
  • LC-MS (Liquid Chromatography-Mass Spectrometry)
  • Creatinine testing – Architect analyzer
  • ELISA, cDNA, DNA cloning
  • Molecular reagents preparation
  • E coli transformations
  • Culturing bacteria
  • Extracting plasmid DNA
  • Nutrient analysis
  • Amino acid analysis
  • Scientific writing
  • Statistical data analysis

References

Available upon request

Publications

Manda, Raghavendra Reddy, Venkata Avinash Addanki, Anita Giabardo, Joshua Benjamin, Mohammad Jonaid Hossain, Sayesha Khanna, Manoj Gaddam, Ravindra Kumar, Seweta Srivastava, Soil Health Management and Microorganisms: Recent Development, Detection, Diagnosis and Management of Soil-borne Phytopathogens, 437-493, Springer Nature Singapore, 2023

Timeline

Clinical Laboratory Technician II (CLIA-certified)

Valley Medical and Wellness
10.2024 - Current

Microbiologist

Transparent Testing, LLC.
04.2024 - 09.2024

Quality Control Laboratory Technician

Laird Family Estate
09.2023 - 03.2024

Graduate Research Assistant

Texas A&M University-Kingsville Citrus Center
01.2022 - 08.2023

MS - Plant and Soil Sciences

Texas A&M University-Kingsville

Bachelor of Science - Agriculture (Honours)

Lovely Professional University

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