Riko Iyogi

To apply for the position of staff member at the Box Office, I'd say my biggest strengths are being inclusive, organized, and empathetic. I really love connecting people from different backgrounds and helping everyone feel comfortable and supported. I've had some fun leadership experiences. I started and captained an intramural volleyball team called "Happirio". As captain, I planned practice, managed schedules, and kept everyone motivated. I also started and direct an original musical project called "Candy Smile". It's a two to three project that brings international and domestic students together through musical performance. These experiences taught me how to lead with teamwork, creativity, and positivity. My goal at MNSU is to help students feel welcomed, supported, and not alone. I want them to look back at their time here and say " It was fun. I felt comfortable. I belonged".As a student who will work at box office I want to create a commuunity where people can smile, grow, succed together, and feel valued for who they are.

• Testing & Improving MULDI-TOF Accuracy
• Riko Iyogi
• UGR 214
• Rembrandt Half Jenny Anderson
• Mass spectrometry is an analytical technique in which samples are ionized into charged molecules and ratio of their mass-to-charge (m/z) can be measured. In MALDI-TOF mass spectrometry, the ion source is matrix-assisted laser desorption/ionization (MALDI), and the mass analyzer is time-of-flight (TOF) analyzer. MALDI is a soft ionization that involves a laser striking a matrix of small molecules to make the analyte molecules into the gas phase without fragmenting or decomposing them. Some biomolecules are too large and can decompose when heated, and traditional techniques will fragment or destroy macromolecules. MALDI is appropriate to analyze biomolecules like peptides, lipids, saccharides, or other organic macromolecules. In this world, there are different yeast, so it may cause different accuracy in MULDI-TOF research.
• Research questions: If researchers use different yeasts, MULTI-DOF may not be accuracy
• Hypothesis and Prediction: If different amounts of cells which means the amount is lower than 0.01 g are tried in MULTI-DOF, then it would be the best signal for MULDI-TOF accuracy.
• As a prediction, we used 0.01g of cells and put them in the sample with the HCCA matrix in MULTI-DOF. The high wave in MULDI-TOF means more accuracy in MULDI-TOF. As production, when we compare the data of cells in 0.0035g and 0.0127g, the data’s wave of 0.0035g is higher than the data of 0.0127g which means less than 0.01g is more likely to be accurate.
• Methods: a Prepare yeasts or bacteria samples (Steak Plates) b Measure the mass of them as 0.01g c Put them on the sample with HCCA matrix d Use MULDI-TOF e Analysis the data
• Supplies: Yeasts, Bacteria, MULTI-DOF machine and craft brewing laboratory member(Jenny)
• Timeline: For students, every weeks in the spring quarter meet in the laboratory (Craft brewing) and continue research and analysis about MULTI-DOF accuracy. After that, the last 3 weeks will be focused on making a group presentation. Laboratory meeting, it’s up to Jenny, Rembrandt and the student’s schedule.
• Work Cite: Espinal a, et al. “Rapid and Accurate Identification of Genomic Species from the Acinetobacter Baumannii (AB) Group by MALDI-TOF MS.” Clinical Microbiology and Infection, Elsevier, 13 Dec. 2014, www.sciencedirect.com/science/article/pii/S1198743X1460745X.
• Singhal, Neelja, et al. “MALDI-Tof Mass Spectrometry: An Emerging Technology for Microbial Identification and Diagnosis.” Frontiers, Frontiers, 21 July 2015, www.frontiersin.org/articles/10.3389/fmicb.2015.00791/full.