Robyn Aune
Crystal
Summary
Detail-oriented Research Associate with 8+ years of experience in mammalian and bacterial cell culture, cell isolations, CRISPR-Cas9 gene editing, molecular biology techniques, and GMP-compliant bioprocessing in regenerative medicine and vaccine production. Proven ability to independently design experiments, troubleshoot protocols, maintain cleanroom environments, and collaborate across teams while ensuring regulatory compliance and high-quality documentation. Adaptable, precise, and skilled at communicating complex scientific information to drive successful biotechnology outcomes.
Overview
10
10
years of professional experience
Work History
Research Associate 3
Recombinetics
New Brighton, MN
04.2021 - Current
- Maintain fibroblast and stem cell cultures and perform isolations from porcine fetal/ear tissue to support regenerative medicine projects and establish new cell lines.
- Execute molecular biology workflows including CRISPR-Cas9 gene editing, PCR, gDNA/RNA extractions/quantification, and sample preparation for Sanger sequencing, NGS, and pathogen testing.
- Independently design, conduct, analyze, and troubleshoot experiments; create, approve, and revise protocols in alignment with good laboratory practices.
- Prepare cell culture media/solutions and manage general lab maintenance while conducting literature reviews to optimize methods and resources.
R&D Technician 3
Miromatrix Medical
Eden Prairie, MN
10.2018 - 04.2021
- Cultured and maintained human umbilical vein endothelial cells (HUVECs); seeded cells into decellularized liver scaffolds and monitored bioreactor performance.
- Designed, set up, troubleshot, and maintained bioreactors; analyzed daily metabolite data (Roche Cedex) to detect contaminations and forecast media requirements.
- Created and revised SOPs; trained new team members on procedures and responsibilities.
- Performed organ sectioning for H&E staining, archived lab documentation, and upheld cleanroom protocols to minimize bioburden in FDA 510(k)-classified biologic device manufacturing.
Laboratory Technician
Pharmgate Biologics
St. Paul, MN
01.2017 - 09.2018
- Executed GMP-compliant production of Mycoplasma hyopneumoniae vaccine, including aseptic antigen harvest and storage.
- Maintained suspension cell cultures, prepared buffers/media, and supported product fills/labeling.
- Managed documentation and coordinated with production, regulatory, and supply chain teams to provide production status updates.
Biomanufacturing Scientist
Aldevron
Fargo, ND
07.2016 - 12.2016
- Performed large-scale DNA purification via bacterial resuspension in a high-throughput environment.
- Handled shaker flask inoculation/harvest and documented production activities.
Education
BA - Biology
Minnesota State University Moorhead
Moorhead, MN05-2015
Skills
- Mammalian & Bacterial Cell Culture (including HUVECs, fibroblasts, suspension cultures)
- Cell Isolations (porcine fetal/ear tissue, PBMCs)
- CRISPR-Cas9 Gene Editing & Molecular Biology (PCR, gDNA/RNA extractions, Sanger/NGS prep)
- Aseptic Technique & Cleanroom Maintenance
- GMP Process Implementation & SOP Development/Editing
- Bioreactor Setup, Maintenance & Metabolite Analysis (Roche Cedex)
- Technical Writing, Protocol Design & Laboratory Documentation
- Data Analysis & Troubleshooting
- Software: Geneious Prime, MS Excel, Word, PowerPoint
Timeline
Research Associate 3
Recombinetics
04.2021 - Current
R&D Technician 3
Miromatrix Medical
10.2018 - 04.2021
Laboratory Technician
Pharmgate Biologics
01.2017 - 09.2018
Biomanufacturing Scientist
Aldevron
07.2016 - 12.2016
BA - Biology
Minnesota State University Moorhead